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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Behav Brain Res. 2016 Jan 20;302:213–219. doi: 10.1016/j.bbr.2016.01.041

Figure 5. Day vs. Night alcohol dehydrogenase (ADH) activity in period mutants.

Figure 5

Comparison of ADH activity in 3-day-old wild type (CS), perS and perL flies during middle of light phase (ZT4 and ZT8) and middle of dark phase (ZT16, and ZT20). Each column represents mean ADH activity +/− standard error of at two time points in 3 biological replicates. ADH activity is represented as normalized absorbance units (NAU). NAU was calculated by normalizing the change in absorbance (ΔOD) due to accumulation of ADH-catalyzed NADH with the total protein concentration in each sample (BCA). X-axis is shaded clear and dark to depict light and dark conditions respectively. For each ADH activity assay five male flies were used.