Fig 3. ssUVR induces reductions in histone acetylation in a dose-dependent manner.
(A) Dose-dependent decreases of histone acetylation by ssUVR. HaCaT cells were exposed to a single ssUVR at 3, 6, 12, and 18 J/cm2, and cells were harvested 24 hr after irradiation. (B) The relative intensity of the bands was presented as the ratio of each band versus its corresponding H3. The results from replicate experiments were plotted as the mean ± SD (n = 3). (C) NHEK cells were exposed to a single ssUVR at 12 J/cm2, and cells were harvested at 24 hours after irradiation. The levels of several histone acetylation marks on histone H3 and H4 were analyzed by western blot analysis using specific antibodies. An antibody against histone H3 was used to assess the loading of samples in each lane.