Table 1. Molecular characterization,resistance pattern and MIC values of the antibiotics and inhibitors for the nine M. tuberculosisstrains.
| Antibiotics (μg/ml) | Inhibitors (μg/ml) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | Resistance pattern | Gene mutations | INH | RIF | AMK | CAP | OFX | VP | TZ | CPZ | FPX | HAL |
| H37Rv | Susceptible | None | 0.1 | 1 | 1 | 2.5 | 1 | 256 | 15 | 30 | 30 | 64 |
| H37RvΔkatG | INH | katG deletion | 128 | 1 | 1 | 2.5 | 1 | 128 | 15 | 30 | 30 | 32 |
| 82/09 | INH; RIF–(MDR) | inhA C-15T/S94A; rpoB S531L | 3 | 320 | 1 | 2.5 | 1 | 256 | 15 | 30 | 30 | 64 |
| 149/09 | INH; RIF; AMK; CAP; OFX– (XDR) | inhA C-15T/I194T; rpoB 531L; gyrA D94A; rrs A1401G | 3 | 320 | 640 | 25 | 10 | 256 | 15 | 30 | 30 | 64 |
| 286/09 | INH; RIF; AMK; CAP; OFX–(XDR) | inhA C-15T/S94A; rpoB S531L; gyrA S91P; eis G-10A; tlyA ins GT at pos 755/756 | 20 | 80 | 4 | 25 | 10 | 128 | 15 | 30 | 30 | 32 |
| 69/11 | INH; RIF; AMK; CAP–(MDR) | katG S315T; rpoB S531L; rrs A1401G/wt* | 20 | 320 | 40 | 25 | 1 | 256 | 15 | 30 | 30 | 32 |
| 29/12 | INH; RIF; AMK; CAP; OFX–(XDR) | inhA C-15T/I194T; rpoB S531L; gyrA D94A; rrs A1401G | 3 | 320 | 640 | >25 | 10 | 256 | 15 | 30 | 30 | 64 |
| 269/03 | INH | katG S315T | 10 | 1 | 1 | 2.5 | 1 | 256 | 15 | 30 | 30 | 64 |
| 294/09 | INH | inhA C-15T | 0.4 | 1 | 1 | 2.5 | 1 | 256 | 15 | 30 | 30 | 64 |
Δ, deletion; MDR, multidrug resistant; XDR, extensively drug resistant; INH, isoniazid; RIF, rifampicin; AMK, amikacin; CAP, capreomycin; OFX, ofloxacin; VP, verapamil; TZ, thioridazine; CPZ, chlorpromazine; FPX, flupenthixol; HAL, haloperidol.
*mixed pattern: simultaneous presence of drug susceptible and drug resistant genotype. The lowest concentration tested corresponds to the critical concentration for each antibiotic (see Material and Methods section for details).