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. 2016 Feb 26;11(2):e0150133. doi: 10.1371/journal.pone.0150133

Fig 2. Effect of reduction in the activities of the proteasome and autophagy on the levels of misfolded and total SOD1 in fibroblast lines.

Fig 2

Misfolded and total SOD1 in lysates of fibroblasts were analysed with the 24–39 misELISA and ELISA for total SOD1, respectively. Fibroblast cell lines were cultured in triplicate for 24 h in the absence (blue) or the presence (red) of the proteasome inhibitor bortezomib (A-C; 5 ng/ml), the autophagy inhibitor 3-MA (D-F; 10 mM). Data is expressed as the mean ± SD (n = 3), *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.0001 compared to respective non-treated control (Welch test and two-tailed unpaired t-test).