Reagent | Type | Manufacturer | Catalog # | Comments |
---|---|---|---|---|
A375 | Cells | ATCC | CRL-1619 | DMEM + 10% FBS |
MeWo | Cells | ATCC | HTB-65 | EMEM + 10% FBS |
HCT116 | Cells | ATCC | CCL-247 | McCoy's 5a Medium Modified + 10% FBS |
PLX4720 | Inhibitor | Symansis | SY-PLX4720 | |
PD0325901 | Inhibitor | Symansis | SY-PD0325901 | |
GDC-0879 | Inhibitor | Selleckchem | S1104 | Replaces Genentech and Array BioPharma source |
Fluorescent plate reader | Equipment | BioTek FLx800 | ||
15-cm cell culture plates | Equipment | Corning | 430599 | Original unspecified |
96-well plates | Materials | Corning | 3903 | |
DMEM | Medium | ATCC | 30–2002 | Original unspecified |
EMEM | Medium | ATCC | 30–2003 | Original unspecified |
McCoy's 5a medium modified | Medium | ATCC | 30–2007 | Original unspecified |
Fetal bovine serum (FBS) | Reagent | ATCC | 30–2020 | Original unspecified |
DMSO | Reagent | Fisher | D128–500 | Original unspecified |
Cell Titer Glo kit | Reagent | Promega | G7570 |