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. 2016 Feb 16;5:e09976. doi: 10.7554/eLife.09976
Reagent Type Manufacturer Catalog # Comments
A375 Cells ATCC CRL-1619 DMEM + 10% FBS
MeWo Cells ATCC HTB-65 EMEM + 10% FBS
HCT116 Cells ATCC CCL-247 McCoy's 5a Medium
Modified + 10% FBS
PLX4720 Inhibitor Symansis SY-PLX4720
PD0325901 Inhibitor Symansis SY-PD0325901
GDC-0879 Inhibitor Selleckchem S1104 Replaces Genentech and
Array BioPharma source
Fluorescent plate reader Equipment BioTek FLx800
15-cm cell culture plates Equipment Corning 430599 Original unspecified
96-well plates Materials Corning 3903
DMEM Medium ATCC 30–2002 Original unspecified
EMEM Medium ATCC 30–2003 Original unspecified
McCoy's 5a medium modified Medium ATCC 30–2007 Original unspecified
Fetal bovine serum (FBS) Reagent ATCC 30–2020 Original unspecified
DMSO Reagent Fisher D128–500 Original unspecified
Cell Titer Glo kit Reagent Promega G7570