Figure 1. TRAF2 is not required for TNF-induced NF-κB and MAPK signalling in bone marrow derived macrophages.

(A and B) Western blot analysis of wild type (WT), Traf2^lox/lox Lyz2-Cre (Traf2^LC) and Sphk1^-/- BMDMs treated with TNF (20 ng/ml) for the indicated times. (C) Flow cytometric analysis of WT, Traf2^LCBMDMs treated with TNF (20 ng/ml) ± 100 nM Smac-mimetic (SM) for 24 hr and stained with Propidium Iodide (PI) and analysed by flow cytometry. (D) WT, Traf2^LCmacrophages were treated with TNF (20 ng/ml) or Smac-mimetic (100 nM) for indicated time and supernatants analysed by TNF ELISA. Data are represented as mean ± SEM.

DOI: http://dx.doi.org/10.7554/eLife.10592.003