Table 9.
Molecular markers for cell identification. The respective markers were crossed with cho-1 or unc-17 fosmid reporters to validate cell identification.
| Neuron | Molecular marker |
|---|---|
| Hermaphrodite | |
| ADFL/R | cat-1::GFP (otIs625) 1 |
| AIA L/R | ttx-3 fosmid::GFP (wgIs68) |
| AIN L/R | ttx-3 fosmid::GFP (wgIs68) |
| AIY L/R | ttx-3 fosmid::GFP (wgIs68) |
| ALN L/R | unc-86 fosmid::YFP (otIs337); lad-2::GFP (otIs439) |
| AS1-11 | unc-3 fosmid::GFP (otIs591) |
| ASJ L/R | DiI/DiO staining |
| AVA L/R | glr-1::DsRed (hdIs30); nmr-1::GFP (akIs3) |
| AVB L/R | acr-15::GFP (wdEx290); sra-11::GFP (otIs123) |
| AVD L/R | glr-1::DsRed (hdIs30); nmr-1::GFP (akIs3) |
| AVE L/R | glr-1::DsRed (hdIs30); nmr-1::GFP (akIs3) |
| AVG | odr-2::DsRed (otEx4452); unc-6 fosmid::GFP (otEx6370) |
| AWB L/R | DiI/DiO staining |
| DA1-9 | unc-3 fosmid::GFP (otIs591); acr-2::GFP (juIs14) |
| DB1-7 | unc-3 fosmid::GFP (otIs591); acr-2::GFP (juIs14) |
| DVA | ser-2::GFP (otIs358) |
| HSN L/R | unc-86 fosmid::YFP (otIs337) |
| IL2 D/V L/R (x6) | unc-86 fosmid::YFP (otIs337) |
| PDA | unc-3 fosmid::GFP (otIs591); ace-3/4::GFP (fpIs1) |
| PDB | unc-3 fosmid::GFP (otIs591) |
| PLN L/R | unc-86 fosmid::YFP (otIs337); lad-2::GFP (otIs439) |
| PVC L/R | nmr-1::GFP (akIs3) |
| PVN L/R | 2 |
| PVP L/R | lin-11 fosmid::GFP (wgIs62); unc-30 fosmid::GFP (wgIs395) |
| RIB L/R | 2 |
| RIF L/R | odr-2::DsRed (otEx4452); unc-6 fosmid::GFP (otEx6370) |
| RIH | cat-1::GFP (otIs625) |
| RIR8 | unc-86 fosmid::YFP (otIs337) |
| RIV L/R | unc-42 fosmid::GFP (wgIs173); lad-2::GFP (otIs439) |
| RMD D/V L/R (x6) | glr-1::DsRed (hdIs30) |
| RMF L/R | 2 |
| RMH L/R | 2 |
| SAA D/V L/R (x4) | lim-4::GFP (mgIs19); lad-2::GFP (otIs439) |
| SAB D V L/R (x3) | unc-4::GFP (vsIs45) |
| SDQ L/R | unc-86 fosmid::YFP (otIs337); lad-2::GFP (otIs439) |
| SIA D/V L/R (x4) | ceh-24::GFP (ccIs4595) |
| SIB D/V L/R (x4) | ceh-24::GFP (ccIs4595) |
| SMB D/V L/R (x4) | lim-4::GFP (mgIs19); lad-2::GFP (otIs439) |
| SMD D/V L/R (x4) | lad-2::GFP (otIs439) |
| URA D/V L/R (x4) | unc-86 fosmid::YFP (otIs337) |
| URB L/R | unc-86 fosmid::YFP (otIs337) |
| URX L/R | flp-10::GFP (otIs92); unc-86 fosmid::YFP (otIs337) |
| VA1-12 | unc-3 fosmid::GFP (otIs591); acr-2::GFP (juIs14) |
| VB1-11 | unc-3 fosmid::GFP (otIs591); acr-2::GFP (juIs14) |
| VC1-6 | lin-11::GFP (nIs106); ida-1::GFP (inIs179) |
| Pharyngeal | |
| I1 L/R | 3 |
| I3 (L) | 3 |
| M1 (R) | 3 |
| M2 L/R | 3 |
| M4 (L) | 3 |
| M5 (L) | 3 |
| MC L/R | 3 |
| M | |
| CEM D/V L/R (x4) | pkd-2::GFP (bxIs14) |
| CA1-9 | ida-1 (inIs179) |
| DVE, DVF | 2 |
| HOB | ida-1 (inIs179) |
| PCB, PCC, SPC | 4 |
| PDC, PGA | 2 |
| PVV | 2 |
| PVX, PVY | 2 |
| PVZ | ida-1 (inIs179) |
| R1A, R2A, R3A, R4A, R6A | 2 |
| SPV | 2 |
1Excluded AWA due to lack of overlap of cho-1 fosmid reporter with odr-10::gfp and unc-17 fosmid reporter gfp reporter with gpa-4::mCherry. See Figure 1—figure supplement 1.
2Identified based on position and axonal projections because of the lack of available markers.
3Pharyngeal neurons identified based on axonal projections which are visible with the unc-17 fosmid reporter.