Reagent	Type	Manufacturer	Catalog #	Comments
pRetroX-IRES-ZsGreen1 vector	Plasmid	Produced in Protocol 1
pRetroX- FLAG-GNAO1WT -IRES-ZsGreen1 vector	Plasmid	Produced in Protocol 1
pRetroX-FLAG-GNAO1R243H -IRES-ZsGreen1 vector	Plasmid	Produced in Protocol 1
HMECs	Cell line	ATCC	PCS-600-010	Original product number not specified; Replaces Life Technology brand used in original study
HMEC medium	Cell culture	ATCC	PCS-600-03	Original product number not specified; Replaces Life Technology brand used in original study
HMEC supplement	Cell culture	ATCC	PCS-600-040	Original product number not specified; Replaces Life Technology brand used in original study
Bovine pituitary extract	Cell culture	Life Technologies	13028014
Penicillin/Streptomycin	Cell culture	Applied Biological Materials	G255	Original not specified
Phoenix amphoteric cells	Cell line	ATCC	ATCC CRL-3213	Replaces Orbigen brand used in original study
DMEM	Cell culture	Sigma	11965-092	Original not specified
Fetal bovine serum (FBS)	Cell culture	Life Technologies	12483-020	Original not specified
L-glutamine	Cell culture	Life Technologies	35050-061	Original not specified
Glucose	Cell culture	Life Technologies	A2494001	Original not specified
Lipofectamine 2000	Transfection Reagent	Life Technologies	11668027
Opti-MEM	Transfection Reagent	Sigma-Aldrich	31985070	Original not specified
PBS	Buffer	GIBCO	10010023	Original not specified
0.45 µm syringe filter	Labware	Millipore	SLHV033RB	Original not specified
Trypsin EDTA	Buffer	ABM	TM050	Original not specified
FBS	Buffer	GIBCO	12483	Original not specified
SDS	Chemical	Left to the discretion of the replicating lab
2-mercaptoethanol	Chemical
Glycerol	Chemical
bromophenol blue	Chemical
Tris-HCl	Chemical
Bradford Assay	Detection assay	Sigma	B6916-500 ML	Original not specified
12% SDS-PAGE gel	Western Blot Reagent	Invitrogen	EC60252BOX	Original 4–20%
OptiProtein Marker	Western Blot Reagent	Applied Biological Materials	G252	Original not specified
PVDF membrane	Western Blot Reagent	Biorad	162-0015	Original Nitrocellulose
Skim milk powder	Western Blot Reagent	Fisher Scientific	361021617	Original not specified
1X TBS solution	Buffer	Fisher Scientific	BP2471-100	Original not specified
Anti-FLAG M2 antibody	Antibody	Sigma	F1804
Anti-ß-ACTIN antibody	Antibody	Abcam	Ab8227	Original not specified
Anti-mouse HRP-conjugated secondary antibody	Antibody	Abcam	Ab6728	Original not specified
ECL Reagent A and B	Western Blot Reagent	Applied Biological Materials	G075	Replaces Thermo Fisher brand.
X-ray Film	Western Blot Reagent	Kodak	XBT-1	Original not specified