| Reagent | Type | Manufacturer | Catalog # | Comments |
|---|---|---|---|---|
| HMECs | Cell line | ATCC | PCS-600-010 | Original product number not specified; Replaces Life Technology brand used in original study |
| HMECs transduced with pRetroX-IRES-ZsGreen1-empty vector |
Cell line | Produced in Protocol 2 | ||
| HMECs transduced with pRetroX-FLAG-GNAO1WT-IRES-ZsGreen1 |
Cell line | Produced in Protocol 2 | ||
| HMECs transduced with pRetroX-FLAG-GNAO1 R243H-IRES-ZsGreen1 |
Cell line | Produced in Protocol 2 | ||
| HMEC medium | Cell culture | ATCC | PCS-600-03 | Replaces Life Technology brand used in original study |
| HMEC supplement | Cell culture | ATCC | PCS-600-040 | Original product number not specified; Replaces Life Technology brand used in original study |
| Bovine pituitary extract | Cell culture | Life Technologies | 13028014 | Originl not specified |
| Penicillin/streptomycin | Cell culture | ABM | G255 | Original not specified |
| 6 well plates | Labware | Fisher Scientific | Biolite 12556004 | Original not specified |
| Low melting temperature agar | Cell culture | Bioworld | 40100048-2 | Original not specified |
| Crystal violet | Dye | Left to the discretion of the replicating lab | Not originally used | |
| Methanol (MeOH) | Chemical | |||
| Acetic acid | Chemical | |||
| ImageJ | Software | NIH | Replaces Oxford Optronix GelCount imager and software |
|
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