Reagent	Type	Manufacturer	Catalog #	Comments
D04 cells	Cells	Provided by Chris Schmidt, Queensland Institute of Medical Research (QIMR) Berghofer, Australia
A375 cells	Cells	ATCC
RPMI	Cell culture media	Life Technologies	21875-034
DMEM	Cell culture media	Life Technologies	41966-029
FBS	Reagent	Life Technologies	10270106
35-mm culture plates	Material	Corning	CLS430165	Original not specified
Sorafenib	Drug	Selleckchem	S7397
PD184352	Drug	Selleckchem	S1020
SB590885	Drug	Selleckchem	S2220	*Replaces Plexxion 885-A
DMSO	Reagent	Fisher Scientific	D128-500	Original not specified
PBS	Reagent	Gibco	10010-023	Original not specified
Tris-HCl	Chemical	Specific brand information will be left up to the discretion of the replicating lab and recorded later
NaCl	Chemical
Igepal	Chemical
Na3VO4	Chemical
NaF	Chemical
Leupeptin	Chemical
Bradford Assay	Detection Assay	Bio-Rad Laboratories	5000001		Original not specified
NuPAGE Sample buffer	Buffer	Invitrogen	NP0007		Original not specified
SDS-Page gel (4–12%)	Western blot reagent	Invitrogen	NP0322BOX		Original not specified
Nitrocellulose membrane (iBlot)	Western blot reagent	Invitrogen	IB301002		Original not specified
Ponceau stain	Western blot reagent	Sigma-Aldrich	P7170-1L		Original not specified
Tris	Chemical	Sigma-Aldrich	T6066		Original not specified
Tween-20	Chemical	Sigma-Aldrich	P1379		Original not specified
Mouse α-ppERK1/2	Antibody	Cell Signaling Technology	9106		Replaces Sigma M8159
Rabbit α-ERK1/2	Antibody	Cell Signaling Technology	9102		Replaces Santa Cruz Bio sc-154
HRP-conjugated secondary antibody	Western blot reagent	Bio-Rad	170-5047		Original not specified
ECL Detection Kit	Western blot reagent	Invitrogen	32132		Original not specified

*Suggested as suitable replacement by original authors by personal communication.