Reagent	Type	Manufacturer	Cat. No.		Comments
D04 cells	Cells	Provided by Chris Schmidt, Queensland Institute of Medical Research (QIMR) Berghofer, Australia
RPMI	Cell culture media	Life Technologies	21875-034
FBS	Reagent	Life Technologies	10270106
SB590885	Drug	Selleckchem	S2220	*Replaces Plexxion 885-A
DMSO	Reagent	Fisher Scientific	D128-500		Original not specified
35 mm tissue culture dishes	Materials	Corning	CLS430165		Original not specified
INTERFERin	Reagent	Polyplus Transfection	409-01
CRAF siRNA	siRNA	Synthesis left to the discretion of the replicating lab and will be recorded later			5’-AAGCACGCTTAGATTG GAATA-3’
NRAS siRNA	siRNA	Synthesis left to the discretion of the replicating lab and will be recorded later			5’-CATGGCACTGTACTCT TCTCG-3’
Scrambled siRNA	siRNA	Synthesis left to the discretion of the replicating lab and will be recorded later			5’-AAACCGTC GATTTCACCCGGG-3’
PBS	Reagent	Gibco	10010-023		Original not specified
Tris-HCl	Chemical	Specific brand information will be left up to the discretion of the replicating lab and recorded later
NaCl	Chemical
Igepal	Chemical
Na3VO4	Chemical
NaF	Chemical
Leupeptin	Chemical
Bradford Assay	Detection Assay	Bio-Rad Laboratories	5000001		Original not specified
NuPAGE Sample buffer	Buffer	Invitrogen	NP0007		Original not specified
SDS-Page gel (4–12%)	Western blot reagent	Invitrogen	NP0322BOX		Original not specified
Nitrocellulose membrane (iBlot)	Western blot reagent	Invitrogen	IB301002		Original not specified
Ponceau stain	Western blot reagent	Sigma-Aldrich	P7170-1L		Original not specified
Tris	Chemical	Sigma-Aldrich	T6066		Original not specified
Tween-20	Chemical	Sigma-Aldrich	P1379		Original not specified
Mouse α NRAS (C-20)	Antibody	Santa Cruz Biotechnology	sc-159
Mouse α CRAF	Antibody	BD Transduction Laboratories	610152
Rabbit α ppMEK1/2	Antibody	Cell Signaling Technology	9121
Mouse α ppERK1/2	Antibody	Sigma	M8159
Mouse α tubulin	Antibody	Sigma	T5168
HPR-conjugated secondary antibody	Western blot reagent	Bio-Rad	170-5047	Original not specified
ECL Detection Kit	Western blot reagent	Invitrogen	32132	Original not specified