Western blot antibody multiplexing | ||||
---|---|---|---|---|
POI | Loading control | |||
Combination | Description | Working conc. | Description | Working conc. |
1 | Rabit anti-ppMEK (45 kDa) | 1:1000 | Mouse anti-tubulin (50 kDa) | 1:5000 |
2 | Rabbit anti-ppERK (42, 44 kDa) | 1:1000 | Mouse anti-tubulin (50 kDa) | 1:5000 |