Figure 7. Computational analysis of water entry through the portal lined by A404 (Helix P).
(A) ClC-ec1 structure highlighting the location of the A404 “portal” residue at Helix P. (B) The D417C cross-link does not affect water entry into the pathway connecting Gluin and Gluex. The aggregate number of water molecules entering the region between the two residues was determined as described previously (Han et al., 2014) and compared for wild-type (WT) and cross-linked mutant (D417C) over the same timescales.