Table 1.

D417C activity extrapolated to 0 and 100% cross-link.

DOI: http://dx.doi.org/10.7554/eLife.11189.016

D417C variant	Turnover at 0% cross-link (s-1)	Turnover at 100% cross-link (s-1)
WT	1440 ± 70	280 ± 70
E148A/Y445S (channel-like)	14400 ± 1300	520 ± 5400
E148A	260 ± 20	76 ± 26
Y445S	850 ± 70	-20 ± 105
A404L	140 ± 20	54 ± 17
L361A (Helix N)	360 ± 30	240 ± 30
F357A (Helix N)	84 ± 9	119 ± 8

Values for turnover at 0 and 100% D417C cross-link were estimated from extrapolation of fits to data in Figures 4, 7 and 11. The uncertainties report the 95%confidence interval in the extrapolated values.