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. 2016 Feb 2;5:e10747. doi: 10.7554/eLife.10747

Figure 3. SsrB regulates biofilms by a CsgD-dependent mechanism.

Figure 3.

(A) The SPI-2 needle, ssaC and ssaJ mutant strains were not affected in biofilm formation. (B) Over-expression of csgD from a plasmid (pBR328::csgD) in trans rescued biofilm formation in the ssrB mutant, as measured by crystal violet staining, n = 3. Source data file: Figure 3—source data 1. An estimate of csgD expression by (C) Real-time qRT-PCR showed a significant decrease in csgD transcription in the ssrB null, but not in the D56A SsrB and ssrA mutants. rrsA transcript levels were used as control; n = 2, Mean ± SD, p < 0.0001. Source data file: Figure 3—source data 2 and (D) Immunoblot analysis showing the absence of CsgD in the ssrB null strain in two day old biofilms, using GroEL as a loading control.

DOI: http://dx.doi.org/10.7554/eLife.10747.014

Figure 3—source data 1. Source data for crystal violet staining in Figure 3A and B.
DOI: http://dx.doi.org/10.7554/eLife.10747.015
elife-10747-fig3-data1.xlsx (37KB, xlsx)
DOI: 10.7554/eLife.10747.015
Figure 3—source data 2. Source data for Real-time qRT-PCR in Figure 3C.
DOI: http://dx.doi.org/10.7554/eLife.10747.016
elife-10747-fig3-data2.xlsx (30.3KB, xlsx)
DOI: 10.7554/eLife.10747.016