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. 2015 Jul 6;27(3):804–813. doi: 10.1681/ASN.2014121228

Figure 5.

Figure 5.

The potentiation of TRPM6 by cAMP signaling depends on a serine residue in the C-terminal tail. (A) Localization of the predicted PKA phosphorylation sites along TRPM6. (B) The predicted PKA phosphorylation sites are individually mutated to alanine mutants and tested for their sensitivity to FSK. All mutants except S1252A responded to the treatment with FSK (10 μM, 15 minutes, full symbols, n≥10 per mutants and conditions). The bottom panel illustrates the expression of the tested mutants. (C) Average whole-cell S1252A and S1252D current amplitudes with FSK (10 μM, 15 minutes, n≥11, full bars) or vehicle (EtOH, 0.1% vol/vol, 15 minutes, n≥11, open bars). Both mutants exhibited normal expression (bottom panel). (D) The TRPM6 segment containing serine 1252 does not align with TRPM7. The TRPM6 residue S1252 is indicated by an arrow. *P<0.05. WT, wild type.