Figure 5.

CX3CR1 blockade increases kidney inflammation and reduces IL-1ra production by Ly6C^high monocyte during sepsis. (A) Intracellular production of IL-1ra was evaluated by intracellular cytokine staining gated on CD11b⁺ Ly6G^neg NK1.1^neg Ly6C^high cells from sham-operated (green) and CLP-operated mice (red) 6 hours after surgery. Gray histogram represents isotype staining. (B) Mean fluorescence intensity of IL-1ra intracellular staining was compared between sham-operated (green) and CLP-operated mice (red) treated or not treated with F1 (purple), 6 hours after surgery (n=6 sham, F1, and 12 WT CLP from two independent experiments; ANOVA t test was used; *P<0.05; ***P<0.001). Kidneys from CLP-operated mice treated with PBS (control) or F1 were extracted 6 hours after CLP and were evaluated by quantitative PCR for (C) TNF-α production, (D) TGF-β production, and (E) IL1ra production. Results are represented as fold increase of CLP-operated mice (n=6 in each groups from two independent experiments; t test was performed; P value are indicated).