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INS-1 β cells were transfected with miRNAs for 24 hours and then were harvested for Q-RTPCR and western blot. (A) Q-RTPCR results. (B) Western blot results. (C) Compare of the seed sequences binding by miR126 on the IRS-1 and IRS-2 3’-UTR region. Nonspecific sequence (NC), miR-126 mimic (126M), miR-126 inhibitor (126I).
