Fig 7. Adjuvanticity of AmpB in a standardized rabbit model of immunization.
Cohorts of adult female New Zealand white rabbits (n = 4) were immunized intramuscularly in the flank region with 10 μg of CRM197 in 0.2 mL of saline (unadjuvanted control) or 10 μg of CRM197 in 0.2 mL of saline plus 100 μg of AmpB-pyridoxal phosphate adduct without any other excipients. Other TLR-active candidate adjuvants were used as comparators. Preimmune test-bleeds were obtained on day 0, and animals were immunized on days 1, 15, and 28. A final bleed was obtained on day 38. CRM197-specific ELISAs were performed using automated liquid handling methods and are depicted as log10 (immune/preimmune) titers.