Skip to main content
. 2016 Feb 26;11(2):e0150263. doi: 10.1371/journal.pone.0150263

Fig 4. Hand1 inhibits the Ihh promoter through Runx2 transactivation.

Fig 4

(A) qPCR analysis of Hand1 (a), Ihh (b), and Runx2 (c) transcripts in ATDC5 cells stably transfected with an empty vector (Control) or Hand1 expression vector. (B) Luciferase assays. COS1 cells were transiently cotransfected with pIhh-luc reporter and the indicated expression vectors. Luciferase data (b) is shown as a percentage of Runx2 activation (normalized to 1.0). The data represent the mean ± SD. (C) Model for transcriptional regulation of endochondral ossification by Hand1. Hand1 inhibits Runx2-dependent Ihh expression, which normally promotes Runx2 expression in the perichondrium and the periosteum, which, in turn, is required for osteoblast differentiation.