Skip to main content
. 2016 Feb 26;11(2):e0149965. doi: 10.1371/journal.pone.0149965

Fig 4. MLIF protects SH-SY5Y cells against apoptosis in an eEF1A2-dependent manner.

Fig 4

OGD-exposed SH-SY5Y cells transfected with eEF1A2 siRNA or negative control siRNA (NC) were treated with MLIF (0.1 μg/ml). Cell survival was measured using MTT assay (A). Representative annexin V/PI labeling, assessed by flow cytometry, was used to analyze the ratio of apoptotic SH-SY5Y cells (B, D). Hoechst 33258 staining was used to evaluate the nuclear morphology of SH-SY5Y cells (C, E). Data are expressed as the mean ± SEM. Results were analyzed using one-way ANOVA; n = 3. **P< 0.01 or *P< 0.05, OGD group vs. control group or MLIF group; ##P < 0.01 or #P< 0.05, eEF1A2 siRNA group vs. NC group.