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. 2016 Feb 26;11(2):e0149889. doi: 10.1371/journal.pone.0149889

Fig 3. Antibodies to Vtp in sera from infected mice with different serotypes of B. hermsii.

Fig 3

Panel A. Dot blot analysis of binding of antibodies in pooled sera from mice infected for 6 weeks with either serotype 7 or serotype 19 to recombinant Vtp proteins from either strain HS1 or CC1 of B. hermsii. Purified proteins at a concentration of 20 μg/ml were spotted onto nitrocellulose membranes in duplicate and then incubated with sera. Panel B. Western blot analysis of pooled week-6 sera from infected mice to recombinant Vtp proteins at concentrations of 50, 5, or 0.1 μg/ml. Molecular weight standards in kilodaltons are shown on the left.