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Hypothalamic knockdown of Bdnf or Vgf in EE. microRNA targeting Bdnf blocked EE-induced Bdnf and Vgf up-regulation (A) and decrease of adiposity (B), n = 8 per group. Cre-loxP-mediated knockdown of Vgf failed to block the EE-induced Bdnf up-regulation (C) or leanness (D). n = 5 per group. Con, control housing; EE, EE housing; rWAT, retroperitoneal WAT; iWAT, inguinal WAT. Data are mean ± SEM; *, P < .05.
