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. 2016 Jan 8;157(3):1307–1314. doi: 10.1210/en.2015-1821

Figure 1.

Figure 1.

Schematic of the SNaPP for global proteomics of nanogram quantities. Autosampler injects protein sample to 25 μL sample loop at valve A. After injection, valve A position is then switched, and nanopump 1 carries out immobilized trypsin digestion at 200 nL/min with 5% acetonitrile in 95% 50 mM Tris (pH 8) for 75 minutes at approximately 70 bars. Digested peptides are loaded directly on SPE after immobilized trypsin digestion. Valve C then switches position and the capillary pump carries out desalting with nanopure water with 0.1% formic acid at 2.5 μL/min flow rate for 20 minutes. After desalting, valve C then switches position and nanopump 2 carries out peptide separation with a 100-minute reversed-phase gradient from 8% to 75% mobile phase B. Mobile phase A consists of nanopure water with 0.1% formic acid, and mobile phase B consists of acetonitrile with 0.1% formic acid.