Figure 6.

Cort inhibits pituitary gene expression at the time of the LH surge. Quantitative RT-PCR analysis of Lhβ (A) and Gnrhr (B) mRNA was performed on individual mouse pituitary glands collected at the time of the expected LH surge (6 pm) from female mice exposed to a surge-inducing estradiol stimulus, as well as a pellet containing cholesterol (Veh) or Cort n = 6 per group. Ct values for Lhβ and Gnrhr were compared with Gapdh using the 2^−ΔΔCt method and analyzed by one-way ANOVA. Data are represented as mean fold change ± SEM. *, P < .05, Veh vs Cort.