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. Author manuscript; available in PMC: 2017 Feb 25.
Published in final edited form as: Cell. 2016 Feb 25;164(5):896–910. doi: 10.1016/j.cell.2015.12.057

Figure 3. Mitochondrial p62 recruitment is Parkin dependent.

Figure 3

(A) Intracellular distribution of p62, polyubiquitin (poly-Ub) and mitochondria (Mitotracker) in LPS-primed WT BMDM stimulated with NLRP3 agonists determined by confocal microscopy. Scale bars: 5 μm. (B) Mitochondrial poly-Ub decoration examined by confocal microscopy in LPS-primed Park2+/+ or Park2−/− BMDM stimulated with NLRP3 agonists. Scale bars: 5 μm. (C) Mitochondrial recruitment of p62 in LPS-primed WT (shCtrl) or Parkin-deficient (shPark2) iBMDM stimulated with NLRP3 agonists. Scale bars: 5 μm. (D) Subcellular distribution of human p62 in LPS-primed, NLRP3 agonist stimulated p62-deficient BMDMs transduced with human p62-full length or p62(ΔUBA) constructs. Scale bars: 5 μm.