. Author manuscript; available in PMC: 2017 Jan 1.

Published in final edited form as: Immunol Rev. 2016 Jan;269(1):145–161. doi: 10.1111/imr.12344

Table 1.

Phenotypes of genetically altered mice with presumed alterations in the CD22 pathway

		B-cell populations			B-cell surface phenotype			Signalling (anti-IgM)			Mouse

Mouse strain	Genetic Background	MZ	B1	Recirc.	sIgM	MHC II	CD22	Ca²⁺	Prolif.	Apop.	Serum IgM	Td	Ti	AutoAb	Reference

CD22^−/−	mixed 129 × B6 (from 129 ES cells)	↓	↑	↓	↓	↑	n.a.	↑	↓	↑	↑	← →	↓	Yes (1 of 3 lines)	46, 133, 134
CD22^−/−	B6, (from B6 ES cells)	↓	← →	↓	↓	↑	n.a.	↑	← →	↑	↑ (> 5 mo)	← →	↓	No	30

CD22Δ1-2	Backcrossed to B6 for 6 generations.	↓	↑	↓	↓	↑	↓	← →	↓	n.d.	n.d.	n.d.	n.d.	n.d.	55
CD22AA	Backcrossed to B6 for 6 generations.	↓	↑	↓	↓	↑	↓	← →	↓	n.d.	n.d.	n.d.	n.d.	n.d.	55
CD22^a.B6	B6 (congenic with NZB CD22a allotype)	← →	n.d.	n.d.	↓	↑	↓	← →	↓	n.d.	n.d.	n.d.	n.d.	Yes (Coombs Ab)	65
CD22 R130E	B6	↓	← →	↓	n.d.	n.d.	← →	↓	n.d.	n.d.	← →	← →	← →	n.d.	39
CD22 ITIM mutant	B6	↓	← →	↓	n.d.	n.d.	← →	↑	n.d.	n.d.	↑	↑	← →	n.d.	39

ST6Gal I^−/−	Backcrossed to B6 for 6 generations from 129.	↓	← →	↓	↓ ↓	← →	↓	↓	↓	n.d.	↓	↓	↓	n.d.	65, 135, 136
ST6Gal I^−/− × CD22^−/−	Backcrossed to B6 for 6 generations from 129.	↓	← →	↓	↓ ↓	n.d	n.a.	↑	↓	n.d.	n.d.	n.d.	n.d.	n.d.	137

SIAE−/−	mixed 129 × B6 (from 129 ES cells)	↓	n.d.	↓	← →	n.d	← →	↑	↓	↑	↑	↓	↑	Yes	19
CMAH−/−	2 lines: B6, mixed 129/B6	↓	n.d.	↓	← →	← →	← →	↑	↑	n.d.	← →	← →	↑	n.d.	91, 138

Note: This an expanded and updated version of a summary table originally presented in a review by Smith et al (11). Td = T-dependent; Ti = T-independent; AutoAb = autoantibody; B6 = C57/BL6; sIgM = surface IgM; n.d = not determined; n.a. = not applicable.