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. 2016 Feb 27;15:124. doi: 10.1186/s12936-016-1177-x

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© Kumar et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Demonstration of representative nested PCR amplification to detect P. vivax (a) and P. falciparum (b): a Lane 1,2,3,4 An stephensi (Mosquito sample ID, ST24, ST31, ST32, ST54) positive for P. vivax; Lane 5 An. subpictus positive for P. vivax (SP12); Lane 6 100 bp DNA ladder b Lane 1 100 bp DNA ladder, Lane 2,3,4,6,8,9,11,12 (Negative, Sample ID, SP315, SP322, SP325, SP369, SP386, SP389, SP392, SP 399). Lane 5, 7 are positive for An. subpictus (SP328, SP371) and Lane 10 is positive for P. falciparum in An. stephensi (ST298)