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. 2016 Feb 26;15:46. doi: 10.1186/s12934-016-0440-8

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© Ignea et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Optimization of 11β-hydroxy-manoyl oxide production in yeast cells. a Bar chart depicting the titers of manoyl oxide and 11β-hydroxy-manoyl oxide obtained using the different strains developed in this study. b Efficiency of conversion of manoyl oxide to 11β-hydroxy-manoyl oxide by CYP76AH24 in the different yeast strains developed. The efficiency of conversion is calculated as the ratio of 11β-hydroxy-manoyl oxide titer to the sum of manoyl oxide and 11β-hydroxy-manoyl oxide titres, expressed as percentage