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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Clin Exp Allergy. 2015 May;45(5):940–952. doi: 10.1111/cea.12481

Fig. 4.

Fig. 4

RELM-β promotes proliferation of primary human lung and MRC5 fibroblasts, myofibroblast differentiation and extracellular matrix protein production in vitro. (a and b) RELM-β-induced proliferation (48 h) of primary human lung fibroblasts and MRC5 cells (a: MTT assay, b: BrdU assay). (c and d) RELM-β-induced production of fibronectin mRNA (c: real-time PCR, 24 h) and protein (d: Western blot, 48 h) by primary human lung fibroblasts. (e) RELM-β-induced production of collagen I protein (48 h, primary human lung fibroblasts). (f) Western blotting of α-SMA protein induced by cultured primary human lung fibroblasts (48 h) RELM-β, TGF-β1 (positive control), IL-4 and IL-13 (all 10 ng/mL). (g) A blocking anti-TGF-β did not significantly reduce RELM-β-induced production of α-SMA protein. Data are expressed as the mean ± SEM of at least four independent experiments. *P < 0.05 vs. control (Dunnett's test).