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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Clin Exp Allergy. 2015 May;45(5):940–952. doi: 10.1111/cea.12481

Fig. 6.

Fig. 6

Effects of RELM-β on collagen I, TGF-β1, TGF-β2, laminin α1 and Hapl1 expression were associated with ERK1/2 phosphorylation. (a) Cultured human lung fibroblasts (cell line MRC5) were treated with different concentrations (0, 1, 10 and 100 ng/mL) of RELM-β for 24 h (real-time PCR on expressed RNA) and 48 h (Western blot or ELISA). Data are expressed as the mean ± SEM from at least four independent experiments. *P < 0.05 vs. medium control (Dunnett's test). (b) Phosphorylated p44/p42 (pERK1/2) protein was detected using Western blotting of cellular extracts obtained 10 or 30 min after stimulation with 1, 10 and 100 ng/mL of RELM-β. In the corresponding bottom panel, blots have been probed with antibody to total ERK to show equivalent total protein loading. (c) Blockade of endogenous TGF-β using an anti-TGF-β antibody did not significantly alter RELM-β-induced phosphorylation of pERK1/2. Data are presented as the mean ± SEM of four experiments. *P < 0.05 vs. control (Dunnett's test).