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. Author manuscript; available in PMC: 2016 Nov 15.
Published in final edited form as: J Immunol. 2015 Sep 30;195(10):4913–4921. doi: 10.4049/jimmunol.1500953

Figure 4. SARM1 is required for TLR7/TLR9-mediated neuronal apoptosis.

Figure 4

(A–C) Primary cortical neurons from wildtype (C57BL/6) or Sarm1 −/− mice were stimulated with (A) TLR7/TLR9agonists (B) Kainic acid or (C) NMDA and analyzed for cell death at (A) 72 hps or (B–C) 18 hps. (A) Wildtype, but not SARM1−/− neurons underwent apoptosis following TLR7/TLR9 stimulation.. Data are the mean ± SD for 4–12 samples per group and are representative of 2–3 replicate experiments. (B–C) Wildtype and SARM1−/− neurons were treated with two-fold dilutions of (B) kainic acid or (C) NMDA. No statistical difference was observed between wildtype and SARM1−/− neurons for either drug. Data are the mean ± SD for 6 samples per group per concentration. Statistical analysis was completed with a two-way ANOVA with a Bonferroni’s multiple comparison post-test. *** P<0.001.