Fig. 11. Myo1F and Myo1E localize to the interior of actin waves.
Cells were cotransfected with GFP-Myo1F and RFP-lifeact (A–C) or with RFP-Myo1E and GFP-Myo1B (D). Actin waves were induced with 1 μM latrunculin. (A) Myo1F-null cells expressing Myo1F and lifeact. (B) Myo1B-null cells expressing Myo1F and lifeact. (C) AX2 cells expressing Myo1F and lifeact, snapshots of wave propagation taken at times indicated in the panel. (D) Myo1B-null cells expressing Myo1E and Myo1B. Images of live cells are shown. Arrows point to actin waves. Bars are 10 μm.