GFP-Myo1A and the F-actin marker, RFP-lifeact, were coexpressed in Myo1A-null cells. Myo1A is present at: (A) the plasma membrane of non-motile cells, (B) the sites of cell-cell contacts, (C) regions of cell-cell contact between chemotaxing cells, (D) in the cortex of pseudopods of randomly moving cells, and (E) at the front of chemotaxing cells. All the above localizations are similar to that for Myo1B. Panel A shows fixed cells, all other panels show live cells. Arrows point to the structures of interest. Bars are 10 μm.