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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Cytoskeleton (Hoboken). 2016 Feb 22;73(2):68–82. doi: 10.1002/cm.21275

Fig. 4. Myo1A is absent from macropinocytic protrusions.

Fig. 4

GFP-Myo1A or GFP-Myo1B was co-expressed with RFP-lifeact in Myo1A-null and Myo1B-null cells, and their distributions observed in live cells undergoing macropinocytosis. (A) Myo1A does not localize to macropinocytic protrusions in Myo1A-null cells. (B) Myo1B does localize to macropinocytic protrusions in Myo1A-cells. (C) Myo1A does not localize to macropinocytic protrusions in Myo1B-null cells. (D) Myo1A does localize to a pseudopods but not to macropinocytic cups in Myo1A-null cells. (E) Myo1B localizes to pseudopod and to a macropinocytic cup in a Myo1A-null cell. (F) Formation of a macropinocytic cup in a Myo1B-null cell coexpressing Myo1A and lifeact. Images were taken at times indicated in the figure. Myo1A does not localize to the cup. (G) Formation of a macropinocytic cup in a Myo1B-null cell coexpressing Myo1B and lifeact. Myo1B does localize to the cup. Images were taken at times indicated in the figure. Arrows point to macropinocytic protrusions. Bars are 10 μm.