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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Cytoskeleton (Hoboken). 2016 Feb 22;73(2):68–82. doi: 10.1002/cm.21275

Fig. 6. Plasma membrane localization of Myo1A requires the BH site.

Fig. 6

GFP-Myo1A-BH-Ala and RFP-lifeact were coexpressed in Myo1A-null cells. (A) BH plots of Myo1A and Myo1A-BH-Ala mutant run with window 19. The position of the BH peak is marked with an arrow. The regions with BH values above the horizontal line at 0.6 are considered to be positive BH peaks and the mutated BH site in the tail is marked with an arrow. (B) Myo1A-BH-Ala does not localize to the plasma membrane of non-motile cells. (C) Myo1A-BH-Ala does not localize to the sites of cell-cell contacts of non-motile cells. (D) Myo1A-BH-Ala localizes to pseudopods. (E) Myo1A-BH-Ala localizes to the front of chemotaxing cells. (F) Myo1A-BH-Ala does not localize to actin waves. (G) Myo1A-BH-Ala does not localize to macropinocytic cups. Arrows point to the structures of interest. (B) shows fixed cells, and (C)–(G) show live cells. Bars are 10 μm.