GFP-Myo1F and RFP-Myo1E were coexpressed with RFP-lifeact or RFP-Myo1E, respectively, in Myo1B-null cells. Myo1F and Myo1E, as well as Myo1B, localize to: (A, B) the plasma membrane, (C, D) sites of cell-cell contacts, (E, F) sites of cell-cell contact between chemotaxing cells, (G, H) pseudopods, and (I, J) the front of chemotaxing cells. (A and B) show fixed non-motile cells, and (C–J) show live cells. Arrows point to the sites of interest. Bars are 10 μm.