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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Cytoskeleton (Hoboken). 2016 Feb 22;73(2):68–82. doi: 10.1002/cm.21275

Fig. 9. Localization of Myo1F in macropinocytic structures.

Fig. 9

GFP-Myo1F or GFP-Myo1B were coexpressed with RFP-lifeact in Myo1B-null cells. Images of live cells were taken at the times indicated in the panels. (A) Localization of Myo1F during formation of various kinds of extensions. Column (a) flat protrusion turning into a macropinocytic cup. Column (b) flat protrusion disappearing without turning into a macropinocytic cup. Column (c) macropinocytic cup turning into a flat protrusion. (B) Localization of Myo1F and F-actin during internalization of a pinocytic vesicle. (C) Localization of Myo1B and F-actin during internalization of a pinocytic vesicle. Arrows point to the locations of interest (see text). Bars are 10 μm.