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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Prog Retin Eye Res. 2015 Sep 4;51:69–89. doi: 10.1016/j.preteyeres.2015.09.002

Figure 10. RPE Immunoproteasome induction by conditions of stress.

Figure 10

Immunoproteasome content was upregulated in cultures of WT murine RPE following exposure to (A) chronic, low levels of hydrogen peroxide (0.5 mM), (B) TNFα (1 ng/ml) to mimic the inflammatory response, and (C) tunicamycin (5 μg/ml) to induce ER stress. Blots show antibody reaction to immunoproteasome subunits LMP2 and LMP7. Total proteasome content was estimated from reactions of alpha subunits (α6, α7), which are present in all proteasome subtypes. (A, B) Graph and numbers below blots indicate the densitometry of immune reactions relative to untreated controls. (C) Graph shows the dose-dependent response of RPE cell viability for WT and cells lacking the LMP7 and MECL-1 (L7M1) immunoproteasome subunits. Unpublished data.