Figure 7.

mRNA expression levels of mTORC1 and mTORC2 in nondifferentiated SH-SY5Y cells (a) and mRNA expression levels of mTORC1 and mTORC2 in differentiated SH-SY5Y cells (b). For neuronal differentiation, SH-SY5Y cells were treated with 10 μM retinoic acid for three days before exposure to MPP⁺. The control groups for nondifferentiated and differentiated SH-SY5Y cells were treated with the same medium without MPP⁺. Real-time quantitative PCRs were repeated three times in triplicate measurement. Data are expressed as relative to a control and shown as mean ± SEM. ∗ indicates P < 0.05 versus control of each group.