Table 5 . Stability study of bosentan in biological samples .
| Sample |
Concentration
(µg/ml) |
Freeze-thaw stability | Room temperature stability | 4 ˚C | |||
| Concentration found(µg/ml) |
Accuracy
(%RE) |
Concentration found(µg/ml) |
Accuracy
(%RE) |
Concentration found (µg/ml) |
Accuracy
(%RE) |
||
| Plasma | 1.0 | 1.1 | 9.0 | 1.1 | 5.0 | 1.1 | 12.0 |
| 2.0 | 2.1 | 4.0 | 2.1 | 4.5 | 2.1 | 5.5 | |
| 3.0 | 3.1 | 1.7 | 3.1 | 3.4 | 3.2 | 5.0 | |
| EBC | 1.0 | 1.0 | 3.0 | 1.0 | 4.0 | 1.1 | 9.0 |
| 2.0 | 2.0 | 1.5 | 2.1 | 5.0 | 2.1 | 4.0 | |
| 3.0 | 3.0 | 1.3 | 3.1 | 3.5 | 3.1 | 4.6 | |
| Urine | 1.0 | 1.1 | 7.0 | 1.1 | 5.0 | 1.1 | 10.0 |
| 2.0 | 2.1 | 4.0 | 2.1 | 2.5 | 2.0 | 2.0 | |
| 3.0 | 3.1 | 4.0 | 3.1 | 2.7 | 3.1 | 3.7 | |
Three freeze–thaw cycles; room temperature 12 h; and 4 °C stability data for studied drugs in urine.