Figure 2.

CpG‐specific methylation levels. Boxplots show significant DNA methylation changes of the single CpG sites between F1_C (white boxes) and F1_H (gray boxes). Scaffolds (scaff) are indicated for the respective gene and genomic CpG positions for each CpG site. Only positions with a coverage ≥ 5 in the respective individual at the certain CpG position are incorporated (n = x). Stat3 is generally higher methylated in liver samples of F1_H compared with F1_C in all CpGs but CpG 5. Those results are similar to those of Ctsz which shows lower methylation in F1_H only in CpG 11. In contrast, methylation levels of Sirt6 varied more frequently in F1_H compared with F1_C. Here, hypomethylation was detected at CpG 1, 2, 4, and 5 and hypermethylation at CpG 3 and CpG 6.