Table 1.

Gene primer sequences and annealing temperatures

Gene	Gene name	Sequence forward primer	Sequence reverse primer	T a [°C]a
Stat3	Signal Transducer and Activator of Transcription 3	AAGTTCACATT|CTTGGGGTT	CTTCGAGACTGAGGTTTACC	50.2
Ctsz	Cathepsin Z	CTTGATGTTGATGCGATCTG	ATGAATACCTGACACCATCG	53.3
Sirt6	Sirtuin 6	GCATCAGTACTGCCTCAG	CTCGAAGGTGGTGTCGAA	52.5
Hmbs	Hydroxymethylbilane synthase	CTTGACACTGCACTGTCCAAGAT	GCAGATGGCTCCAAT GGTG	58.8
B2 m	Beta‐2‐microglobulin	CAAGTGTATTCCCGTCACCC	GTCTGACATCTCCACATTGTCTATC	55.9
Gapdh	Glyceraldehyde‐3‐phosphate dehydrogenase	CTCACTTGAAGGGTGGTGC	CAACCGACACATTAGGTGTG	55

^a T _a, annealing temperature.