Table 5.
Colonization, invasion and transcytosis of polar NHBE
| Average number of bacteria at the time indicated | ||||
|---|---|---|---|---|
| R2866wt | 12hrs | 24hrs | 168hrs | 336hrs |
| Apical supernatant (CFU) | 1.55×107 | 2.15×108 | 9.25×107 | 2.45×106 |
| Invaded (CFU) | 0 | 2.04×104 | 1.50×105 | 2.25×106 |
| Transcytosed (CFU) | 0 | 1.20×102 | 8.42×107 | 1.83×107 |
| R2866 modA10::kan | ||||
| Apical supernatant (CFU) | 6.82 ×108 | 4.40×108 | 5.20×108 | 6.82×107 |
| Invaded (CFU) | 0 | 5.28×105* | 1.69×107* | 4.95×106 |
| Transcytosed (CFU) | 0 | 8.75×106* | 1.08×108* | 1.73×107 |
Initial inoculum of R2866 was 5.87×103 CFU, while for strain R2866modA10::kan it was 7.51 ×103
*
Statistical significance compared to R2866wt; P = <0.05