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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2015 Aug 28;164:72–78. doi: 10.1016/j.jsbmb.2015.08.023

Figure 5. Western blots showing the upregulation of two molecular markers of monocytic differentiation (VDR and C/EBP) following the exposure to AraC alone, 1-D2/CA combination, and those added sequentially.

Figure 5

When added alone AraC and D2/CA combination significantly increase VDR, expression and this is enhanced in both cell lines when the 1-D2/CA combination follows AraC treatment, as expected. C/EBP β expression is also increased by AraC, but is enhanced by 1-D2/CA only in U937 cells. The Crk-L signal provided a loading control for the Westerns, which was used to determine the corrected OD for each signal. The bar chart below each blot shows the Mean and SD of replicate blots, #= p< 0.05 vs untreated control; * = p< 0.05 vs AraC alone; n=3.