Table 1.
Primer sequences used for qRT-PCR
| PCR Primer Sequence 5’ → 3” | Melt Curve Temperature | |
|---|---|---|
| PGC-1α1 | F: ATGGAGTGACATCGAGTGTGCT R: GAGTCCACCCAGAAAGCTGT |
58.7°C |
| PGC-1α4 | F: TCACACCAAACCCACAGAGA R: CTGGAAGATATGGCACAT |
60°C |
| IGF-1Ea | F: GCCTGCTCACCTTCACCAGC R: TCAAATGTACTTCCTTCTGGGTCTTG |
83°C |
| IGF-1Eb | F: ATCTACCAACAAGAACACG R: TACTTCCAATCTCCCTCC |
83°C |
| MGF | F: CGAAGTCTCAGAGAAGGAAAGG R: ACAGGTAACTCGTGCAGAGC |
84°C |
| Myostatin | F: CTGTAACCTTCCCAGGACCA R: CCCATCCAAAAGCTCAAAA |
82°C |
| FOXO3A | F: GAACGTGGGAACTTCACTGGTGCTA R: GGTCTGCTTTGCCCACTTCCCCTT |
84°C |
| MuRF1 | F: CTCAGTGTCCATGTCTGGAGGCCGTT R: GGCCGACTGGAGCACTCCTGTTTGTA |
79°C |
| MAFbx | F: CATCCTTATGTACACTGGTCCAAAGA R: TCCGATGATACACCCACATGTTAATG |
82°C |
| GAPDH | F: AGCCACATCGCTCAGACA R: GCCCAATACGACCAAATCC |
60°C |
F: Forward; R: Reverse;