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KLF4 is not required for RGC cell numbers. A, Retinal cross sections were immunostained for Brn3a (orange) and counterstained with DAPI (blue). B, Brn3a+ cells showed no statistically significant difference between WT and KLF4-cKO groups (n.s.; values are reported as the mean ± SD; N = 18). Scale bar, 50 μm.
