Figure 6.

rPepO induces phosphorylation of MAPKs and Akt via TLR2 and TLR4 signaling. (A–D) WT, TLR2 or TLR4 deficient PEMs were incubated with rPepO (10 μg /ml) for the indicated times (0–60 min). Cell lysates were prepared, and western blot analysis was used to determine the phosphorylation of p38, Erk, JNK, and Akt. Representative blots of three independent experiments with consistent outcome are shown. The relative band intensities of three tests are shown in histograms above. The data are shown as the mean ± SD (n = 3). Student's t-test was performed to calculate the statistical significance (^*p < 0.05; ^**p < 0.01; ^***p < 0.001).