Figure 1. Germline VRC01-class antibody-binding to 426c gp120 variants.

(a) ELISA was used to evaluate the binding of mature (left panel) or germline (right panel) VRC01 and NIH45-46 to the indicated Envs lacking NLGS in Loop D and V5. Error bars represent the s.d. from three technical replicates. (b) His-tagged gp120 variants of 426c were immobilized on a Ni-NTA biosensor and binding to 20 μg ml⁻¹ solution of the indicated germline, VRC01 class antibodies was measured by BLI. The mutations tested are indicated on the top of each panel (nomenclature in parentheses corresponds to that in Table 1). BLI traces are representative of at least three independent experimental replicates. Dotted line demarcates the association and dissociation phases.