Table 1. Summary data of isolated cardiomyocyte contraction and relaxation under basal conditions and beta-adrenergic stimulation (isoproterenol, 10−6 M).
Contraction | Relaxation | |||||
---|---|---|---|---|---|---|
%RCL | TTPs | ΔF/F0 | TTPc | Shortening RT50 | Ca Transient RT50 | |
Basal | ||||||
Control | 3.5±0.4 | 344±7 | 1.4±0.1 | 107±2 | 336±11 | 240±7 |
WT | 3.5±0.4 | 355±7 | 1.6±0.1 | 108±2 | 340±8 | 241±5 |
D73N | 2.4±0.2* | 336±9 | 1.6±0.1 | 99±2 | 302±11* | 225±7 |
L48Q | 4.7±0.4* | 332±7 | 1.5±0.1 | 112±2 | 326±11 | 237±9 |
ISO | ||||||
Control | 7.4±0.9 | 212±7 | 4.8±0.3 | 93±2 | 192±10 | 106±4 |
WT | 8.3±1.4 | 228±7 | 4.7±0.2 | 99±2 | 218±18 | 119±4 |
D73N | 4.9±0.7* | 219±9 | 3.3±0.3* | 91±2 | 184±12 | 107±4 |
L48Q | 9.6±1.2 | 221±5 | 4.3±0.2 | 104±2 | 197±15 | 123±4 |
ANOVA, analysis of variance; F/F0, Ca2+ transient amplitude; %RCL, per cent change in resting cell length; RT50, relaxation time to 50%; TTPc, time to peak Ca2+ transient; TTPs, time to peak shortening.
n⩾19 myocytes/group.
*P<0.05 versus other groups using ANOVA and Newman–Keuls pairwise analysis.