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. 2016 Feb 24;7:10794. doi: 10.1038/ncomms10794

Table 1. Summary data of isolated cardiomyocyte contraction and relaxation under basal conditions and beta-adrenergic stimulation (isoproterenol, 10−6 M).

  Contraction Relaxation
  %RCL TTPs ΔF/F0 TTPc Shortening RT50 Ca Transient RT50
Basal
 Control 3.5±0.4 344±7 1.4±0.1 107±2 336±11 240±7
 WT 3.5±0.4 355±7 1.6±0.1 108±2 340±8 241±5
 D73N 2.4±0.2* 336±9 1.6±0.1 99±2 302±11* 225±7
 L48Q 4.7±0.4* 332±7 1.5±0.1 112±2 326±11 237±9
 
ISO
 Control 7.4±0.9 212±7 4.8±0.3 93±2 192±10 106±4
 WT 8.3±1.4 228±7 4.7±0.2 99±2 218±18 119±4
 D73N 4.9±0.7* 219±9 3.3±0.3* 91±2 184±12 107±4
 L48Q 9.6±1.2 221±5 4.3±0.2 104±2 197±15 123±4

ANOVA, analysis of variance; F/F0, Ca2+ transient amplitude; %RCL, per cent change in resting cell length; RT50, relaxation time to 50%; TTPc, time to peak Ca2+ transient; TTPs, time to peak shortening.

n⩾19 myocytes/group.

*P<0.05 versus other groups using ANOVA and Newman–Keuls pairwise analysis.